Quantitative Synthetic Cryptosporidium hominis DNA can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine protozoan load. Preparation includes fragments from 18s rRNA, heat shock protein 70 (hsp70), COWP, GP60, dnaJ-like protein, and LIB13 regions.